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名稱	Recombinant Human IFN-γ
型號
更新時間	2023-09-24
特點	IFN-γ是acid-labile干擾素產生的CD4和CD8 T淋巴細胞激活NK細胞。IFN-γ刺激的淋巴細胞功能的抗菌和抗腫瘤反應包括巨噬細胞、NK細胞和中性粒細胞。人類IFN-γ種專一性和生物活性只有在人類與靈長類動物的細胞。重組人類IFN-γ16.8 kDa蛋白質含有144個氨基酸殘基。使用所有無動物生產試劑。}

詳細內容

一.產品名稱：Recombinant Human IFN-γ

二.Catalog Number:AF-300-02

三.Synonyms:Immune Interferon, type II interferon, T cell interferon, MAF

四.Description:

IFN-γ is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by increasing the surface expression of class I MHC proteins. This promotes the presentation of antigen to T-helper (CD4+) cells. IFN-γ signaling in antigen-presenting cells and antigen-recognizing B and T lymphocytes regulate the antigen-specific phases of the immune response. Additionally, IFN-γ stimulates a number of lymphoid cell functions including the anti-microbial and anti-tumor responses of macrophages, NK cells, and neutrophils. Human IFN-γ species-specific and is biologically active only in human and primate cells. Recombinant human IFN-γ is a 16.8 kDa protein containing 144 amino acid residues. Manufactured using all non-animal reagents.

五.AA Sequence:

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ

六.Source:E.coli

七.Purity:Greater than 98% by SDS-PAGE gel and HPLC analyses.

八.Biological Activity:

Assay #1: Determined by its ability to induce apoptosis in HeLa cells. The expected ED₅₀ for this effect is 5.0-10.0 ng/ml.
Assay #2: The ED₅₀ was determined by a cytotoxicity assay using HT-29 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 10⁷ units/mg.

九.Cross Reactivity:

Monkey, Human, Human + Bacteria, Mouse, Mandarin Fish, Fish + Virus