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當(dāng)前位置:首頁 > 技術(shù)文章 > 無動(dòng)物成分重組蛋白與傳統(tǒng)重組蛋白有什么異同?

無動(dòng)物成分重組蛋白與傳統(tǒng)重組蛋白有什么異同?

2014-08-14 瀏覽次數(shù):2340

   無動(dòng)物成分重組蛋白         傳統(tǒng)重組蛋白
蛋白種屬人、大鼠、小鼠
表達(dá)細(xì)胞原核細(xì)胞原核細(xì)胞為主,真核細(xì)胞為輔
蛋白表達(dá)體系不含任何動(dòng)物成分添加胰蛋白胨或牛血清等
蛋白氨基酸序列*一致
蛋白生物活性*相同
優(yōu)點(diǎn)    與傳統(tǒng)重組蛋白相比,無動(dòng)物成分重組蛋白具有以下優(yōu)勢:
    ? 不會(huì)給培養(yǎng)體系中引入動(dòng)物源性病原體(如瘋牛病病毒等),因此,非
      常適合臨床用的細(xì)胞培養(yǎng)(如細(xì)胞治療等)。
    ? 進(jìn)入動(dòng)物體內(nèi)不會(huì)引起異種排斥和過敏反應(yīng),因此更適合體內(nèi)實(shí)驗(yàn)。
    ? 無動(dòng)物源性蛋白和激素等對(duì)實(shí)驗(yàn)結(jié)果的干擾,因此保證實(shí)驗(yàn)結(jié)果的穩(wěn)
      定、可靠。
舉例無動(dòng)物成分的人IL-2重組蛋白中不會(huì)含有牛的病原體,蛋白(如牛IL-2)和激素等傳統(tǒng)的人IL-2重組蛋白中可能含有牛的病原體,蛋白(如牛IL-2)和激素等

 

      PeproTech現(xiàn)有的無動(dòng)物成分重組蛋白(共82種)

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Activin-AAF-120-14E10ug/100ug/500ug/1mg
Human BAFFAF-310-1320ug/100ug/500ug/1mg
Human BDNFAF-450-0210ug/100ug/500ug/1mg
Human BMP-2AF-120-0210ug/100ug/500ug/1mg
Human BMP-4AF-120-05ET10ug/100ug/500ug/1mg
Human CD40-LigandAF-310-0250ug/100ug/500ug/1mg
Human CNTFAF-450-1320ug/100ug/500ug/1mg
Human EGFAF-100-15100ug/500ug/1mg
Human FGF-acidicAF-100-17A50ug/100ug/500ug/1mg
Human FGF-basicAF-100-18B50ug/100ug/500ug/1mg
Human FGF-4AF-100-3125ug/100ug/500ug/1mg
Human FGF-8 AF-100-2525ug/100ug/500ug/1mg
Human FGF-9AF-100-2320ug/100ug/500ug/1mg
Human FGF-10AF-100-2625ug/100ug/500ug/1mg
Human Flt-3 LigandAF-300-1910ug/50ug/100ug/500ug/1mg
Human G-CSFAF-300-2310ug/100ug/500ug/1mg
Human GDF-3AF-120-2220ug/100ug/500ug/1mg
Human GDNF AF-450-1010ug/100ug/500ug/1mg
Human GM-CSFAF-300-0320ug/50ug/100ug/500ug/1mg
Human Heregulin beta-1AF-100-0350ug/100ug/500ug/1mg
Human IFN-gammaAF-300-02100ug/500ug/1mg
Human IFN-Lambda-1AF-300-02L20ug/100ug/500ug/1mg
Human IGF-IAF-100-11100ug/1mg
Human IGF-IIAF-100-12 50ug/100ug/500ug/1mg
Human IL-1αAF-200-01A10ug/100ug/500ug/1mg
Human IL-1betaAF-200-01B10ug/100ug/500ug/1mg
Human IL-1RaAF-200-01RA100ug/500ug/1mg
Human IL-2AF-200-0250ug/100ug/500ug/1mg
Human IL-3AF-200-0310ug/100ug/500ug/1mg
Human IL-4AF-200-0420ug/100ug/500ug/1mg
Human IL-6AF-200-0620ug/50ug/100ug/500ug/1mg
Human IL-7AF-200-0710ug/100ug/500ug/1mg
Human IL-8 (72a.a.) (CXCL8)AF-200-08M25ug/100ug/500ug/1mg
Human IL-9 AF-200-0910ug/100ug/500ug/1mg
Human IL-10AF-200-1010ug/100ug/500ug/1mg
Human IL-13AF-200-13 10ug/100ug/500ug/1mg
Human IL-15AF-200-1510ug/100ug/500ug/1mg
Human IL-16 (121aa)AF-200-16A10ug/100ug/500ug/1mg
Human IL-17AF-200-1725ug/100ug/500ug/1mg
Human IL-17E AF-200-2425ug/100ug/500ug/1mg
Human IL-21AF-200-2110ug/100ug/500ug/1mg
Human IL-22 AF-200-2210ug/100ug/500ug/1mg
Human IL-33AF-200-33 10ug/100ug/500ug/1mg
Human IL-36γ (IL-1F9)AF-200-36G10ug/100ug/500ug/1mg
Human KGFAF-100-1910ug/100ug/500ug/1mg
Human LIFAF-300-0525ug/100ug/500ug/1mg
Human MCP-1/MCAF (CCL2)AF-300-0420ug/100ug/500ug/1mg
Human M-CSFAF-300-2510ug/50ug/100ug/500ug/1mg
Human B-NGFAF-450-01100ug/500ug/1mg
Human NT-3AF-450-0310ug/100ug/500ug/1mg
Human NT-4AF-450-0410ug/100ug/500ug/1mg
Human PDGF-AAAF-100-13A10ug/100ug/500ug/1mg
Human PDGF-BBAF-100-14B10ug/100ug/500ug/1mg
Human PlGF-IAF-100-0625ug/100ug/500ug/1mg
Human RantesAF-300-0620ug/100ug/500ug/1mg
Human SCFAF-300-0710ug/50ug/100ug/500ug/1mg
Human SDF-1α (CXCL12)AF-300-28A 10ug/50ug/100ug/500ug/1mg
Human SDF-1β(CXCL12)AF-300-28B10ug/50ug/100ug/500ug/1mg
Human TGF-alphaAF-100-16A100ug/500ug/1mg
Human TGF-beta1AF-100-21C10ug/50ug/100ug/500ug/1mg
Human TGF-beta3AF-100-36E10ug/100ug/500ug/1mg
Human TNF-alphaAF-300-01A50ug/100ug/500ug/1mg
Human TPOAF-300-1810ug/50ug/100ug/500ug/1mg
Human Tweak AF-310-0625ug/100ug/500ug/1mg
Human VEGFAF-100-2010ug/100ug/500ug/1mg
Human VEGF 121AF-100-20A10ug/100ug/500ug/1mg
Human VEGF 165AF-100-2010ug/100ug/500ug/1mg
Human VitronectinAF-140-09500ug/1mg
Murine EGFAF-315-09500ug/1mg
Murine FGF-BasicAF-450-3350ug/100ug/500ug/1mg
Murine G-CSFAF-250-0510ug/100ug/500ug/1mg
Murine GM-CSFAF-315-0320ug/50ug/100ug/500ug/1mg
Murine IL-3AF-213-1310ug/50ug/100ug/500ug/1mg
Murine IL-4AF-214-1420ug/50ug/100ug/500ug/1mg
Murine IL-6AF-216-1610ug/100ug/500ug/1mg
Murine M-CSFAF-315-0210ug/50ug/100ug/500ug/1mg
Murine SCFAF-250-0310ug/50ug/100ug/500ug/1mg
Murine TPOAF-315-1410ug/100ug/500ug/1mg
Murine VEGFAF-450-3210ug/100ug/500ug/1mg
Rat GM-CSFAF-400-2320ug/100ug/500ug/1mg
Rat M-CSFAF-400-28 10ug/50ug/100ug/500ug/1mg
Rat TPOAF-400-3410ug/100ug/500ug/1mg

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